bowtie is An ultrafast, memory-efficient short read aligner
Introduction
In this tutorial we learn how to install bowtie on CentOS 8.
What is bowtie
Bowtie, an ultrafast, memory-efficient short read aligner for short DNA sequences (reads) from next-gen sequencers. Please cite B, et al. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10
We can use yum or dnf to install bowtie on CentOS 8. In this tutorial we discuss both methods but you only need to choose one of method to install bowtie.
Install bowtie on CentOS 8 Using dnf
Update yum database with dnf using the following command.
sudo dnf makecache --refresh
The output should look something like this:
CentOS Linux 8 - AppStream 43 kB/s | 4.3 kB 00:00
CentOS Linux 8 - BaseOS 65 kB/s | 3.9 kB 00:00
CentOS Linux 8 - ContinuousRelease 43 kB/s | 3.0 kB 00:00
CentOS Linux 8 - Extras 23 kB/s | 1.5 kB 00:00
CentOS Linux 8 - FastTrack 40 kB/s | 3.0 kB 00:00
CentOS Linux 8 - HighAvailability 36 kB/s | 3.9 kB 00:00
CentOS Linux 8 - Plus 24 kB/s | 1.5 kB 00:00
CentOS Linux 8 - PowerTools 50 kB/s | 4.3 kB 00:00
Extra Packages for Enterprise Linux Modular 8 - x86_64 13 kB/s | 9.2 kB 00:00
Extra Packages for Enterprise Linux 8 - x86_64 24 kB/s | 8.5 kB 00:00
Metadata cache created.
After updating yum database, We can install bowtie using dnf by running the following command:
sudo dnf -y install bowtie
Install bowtie on CentOS 8 Using yum
Update yum database with yum using the following command.
sudo yum makecache --refresh
The output should look something like this:
CentOS Linux 8 - AppStream 43 kB/s | 4.3 kB 00:00
CentOS Linux 8 - BaseOS 65 kB/s | 3.9 kB 00:00
CentOS Linux 8 - ContinuousRelease 43 kB/s | 3.0 kB 00:00
CentOS Linux 8 - Extras 23 kB/s | 1.5 kB 00:00
CentOS Linux 8 - FastTrack 40 kB/s | 3.0 kB 00:00
CentOS Linux 8 - HighAvailability 36 kB/s | 3.9 kB 00:00
CentOS Linux 8 - Plus 24 kB/s | 1.5 kB 00:00
CentOS Linux 8 - PowerTools 50 kB/s | 4.3 kB 00:00
Extra Packages for Enterprise Linux Modular 8 - x86_64 13 kB/s | 9.2 kB 00:00
Extra Packages for Enterprise Linux 8 - x86_64 24 kB/s | 8.5 kB 00:00
Metadata cache created.
After updating yum database, We can install bowtie using yum by running the following command:
sudo yum -y install bowtie
How To Uninstall bowtie on CentOS 8
To uninstall only the bowtie package we can use the following command:
sudo dnf remove bowtie
bowtie Package Contents on CentOS 8
/usr/bin/bowtie
/usr/bin/bowtie-align-l
/usr/bin/bowtie-align-l-debug
/usr/bin/bowtie-align-s
/usr/bin/bowtie-align-s-debug
/usr/bin/bowtie-build
/usr/bin/bowtie-build-l
/usr/bin/bowtie-build-l-debug
/usr/bin/bowtie-build-s
/usr/bin/bowtie-build-s-debug
/usr/bin/bowtie-inspect
/usr/bin/bowtie-inspect-l
/usr/bin/bowtie-inspect-l-debug
/usr/bin/bowtie-inspect-s
/usr/bin/bowtie-inspect-s-debug
/usr/lib/.build-id
/usr/lib/.build-id/00
/usr/lib/.build-id/00/31f9fc30b4b44f84708d02da21f90430c6f1b8
/usr/lib/.build-id/0d
/usr/lib/.build-id/0d/e35faf8ee6b766edf6c3277525d92ffdf02feb
/usr/lib/.build-id/25
/usr/lib/.build-id/25/8072302f5f6386982ef1153942aaad375e02ed
/usr/lib/.build-id/4c
/usr/lib/.build-id/4c/96e7db48d05480a0bf66a70c83ec4a4da9384d
/usr/lib/.build-id/57
/usr/lib/.build-id/57/05b921fefb38e3596086f72493cdd185c44d6f
/usr/lib/.build-id/69
/usr/lib/.build-id/69/b9b39fa724ace66bb9b8cd091f1f453340c359
/usr/lib/.build-id/81
/usr/lib/.build-id/81/a68497086c8c9f50a7f2bfd65d6b31d6f2850e
/usr/lib/.build-id/82
/usr/lib/.build-id/82/7b7d97a43ee77d37508b1749853eb1986d6953
/usr/lib/.build-id/aa
/usr/lib/.build-id/aa/654f3cac011a6f74677434a740e933a333a63c
/usr/lib/.build-id/b0
/usr/lib/.build-id/b0/77db3f90088cedbf577eef9ea0c1795369dbcb
/usr/lib/.build-id/cc
/usr/lib/.build-id/cc/c401b17213a0ee796162a58c3039b8674277c8
/usr/lib/.build-id/df
/usr/lib/.build-id/df/980c30578ec3c5a881a102f59ea51c3ee296f0
/usr/share/bowtie
/usr/share/bowtie/genomes
/usr/share/bowtie/genomes/NC_008253.fna
/usr/share/bowtie/indexes
/usr/share/bowtie/indexes/e_coli.1.ebwt
/usr/share/bowtie/indexes/e_coli.2.ebwt
/usr/share/bowtie/indexes/e_coli.3.ebwt
/usr/share/bowtie/indexes/e_coli.4.ebwt
/usr/share/bowtie/indexes/e_coli.README
/usr/share/bowtie/indexes/e_coli.rev.1.ebwt
/usr/share/bowtie/indexes/e_coli.rev.2.ebwt
/usr/share/bowtie/reads
/usr/share/bowtie/reads/e_coli_1000.fa
/usr/share/bowtie/reads/e_coli_1000.fq
/usr/share/bowtie/reads/e_coli_1000.raw
/usr/share/bowtie/reads/e_coli_10000snp.fa
/usr/share/bowtie/reads/e_coli_10000snp.fq
/usr/share/bowtie/reads/e_coli_1000_1.fa
/usr/share/bowtie/reads/e_coli_1000_1.fq
/usr/share/bowtie/reads/e_coli_1000_2.fa
/usr/share/bowtie/reads/e_coli_1000_2.fq
/usr/share/bowtie/reads/e_coli_1000_interleaved.fq
/usr/share/bowtie/scripts
/usr/share/bowtie/scripts/best_verify.pl
/usr/share/bowtie/scripts/bowtie-hbb.sh
/usr/share/bowtie/scripts/bs_mapability.pl
/usr/share/bowtie/scripts/build_test.sh
/usr/share/bowtie/scripts/colorize_fasta.pl
/usr/share/bowtie/scripts/colorize_fastq.pl
/usr/share/bowtie/scripts/convert_quals.pl
/usr/share/bowtie/scripts/fastq_to_tabbed.pl
/usr/share/bowtie/scripts/gen_2b_occ_lookup.pl
/usr/share/bowtie/scripts/gen_dnamasks2colormask.pl
/usr/share/bowtie/scripts/gen_occ_lookup.pl
/usr/share/bowtie/scripts/gen_solqual_lookup.pl
/usr/share/bowtie/scripts/infer_fraglen.pl
/usr/share/bowtie/scripts/make_a_thaliana_tair.sh
/usr/share/bowtie/scripts/make_b_taurus_UMD3.sh
/usr/share/bowtie/scripts/make_c_elegans.sh
/usr/share/bowtie/scripts/make_canFam2.sh
/usr/share/bowtie/scripts/make_d_melanogaster.sh
/usr/share/bowtie/scripts/make_e_coli.sh
/usr/share/bowtie/scripts/make_galGal3.sh
/usr/share/bowtie/scripts/make_h_sapiens_ncbi36.sh
/usr/share/bowtie/scripts/make_h_sapiens_ncbi37.sh
/usr/share/bowtie/scripts/make_hg18.sh
/usr/share/bowtie/scripts/make_hg19.sh
/usr/share/bowtie/scripts/make_m_musculus_ncbi37.sh
/usr/share/bowtie/scripts/make_mm8.sh
/usr/share/bowtie/scripts/make_mm9.sh
/usr/share/bowtie/scripts/make_rn4.sh
/usr/share/bowtie/scripts/make_s_cerevisiae.sh
/usr/share/bowtie/scripts/mapability.pl
/usr/share/bowtie/scripts/pe_verify.pl
/usr/share/bowtie/scripts/reconcile_alignments.pl
/usr/share/bowtie/scripts/reconcile_alignments_pe.pl
/usr/share/bowtie/scripts/run-hbb.sh
/usr/share/doc/bowtie
/usr/share/doc/bowtie/AUTHORS
/usr/share/doc/bowtie/MANUAL
/usr/share/doc/bowtie/NEWS
/usr/share/doc/bowtie/TUTORIAL
/usr/share/doc/bowtie/VERSION
/usr/share/doc/bowtie/manual.html
/usr/share/doc/bowtie/style.css
/usr/share/licenses/bowtie
/usr/share/licenses/bowtie/GPL.txt
/usr/share/licenses/bowtie/LGPL.txt
/usr/share/licenses/bowtie/LICENSE
References
Summary
In this tutorial we learn how to install bowtie on CentOS 8 using yum and dnf.
