Introduction
In this tutorial we learn how to install berkeley-express on Ubuntu 22.04.
What is berkeley-express
berkeley-express is:
eXpress is a streaming tool for quantifying the abundances of a set of
target sequences from sampled subsequences. Example applications include
transcript-level RNA-Seq quantification, allele-specific/haplotype
expression analysis (from RNA-Seq), transcription factor binding
quantification in ChIP-Seq, and analysis of metagenomic data. It is
based on an online-EM algorithm that results in space (memory)
requirements proportional to the total size of the target sequences and
time requirements that are proportional to the number of sampled
fragments. Thus, in applications such as RNA-Seq, eXpress can accurately
quantify much larger samples than other currently available tools
greatly reducing computing infrastructure requirements. eXpress can be
used to build lightweight high-throughput sequencing processing
pipelines when coupled with a streaming aligner (such as Bowtie), as
output can be piped directly into eXpress, effectively eliminating the
need to store read alignments in memory or on disk.
In an analysis of the performance of eXpress for RNA-Seq data, it was
observed that this efficiency does not come at a cost of accuracy.
eXpress is more accurate than other available tools, even when limited
to smaller datasets that do not require such efficiency. Moreover, like
the Cufflinks program, eXpress can be used to estimate transcript
abundances in multi-isoform genes. eXpress is also able to resolve
multi-mappings of reads across gene families, and does not require a
reference genome so that it can be used in conjunction with de novo
assemblers such as Trinity, Oases, or Trans-ABySS. The underlying model
is based on previously described probabilistic models developed for
RNA-Seq but is applicable to other settings where target sequences are
sampled, and includes parameters for fragment length distributions,
errors in reads, and sequence-specific fragment bias.
eXpress can be used to resolve ambiguous mappings in other
high-throughput sequencing based applications. The only required inputs
to eXpress are a set of target sequences and a set of sequenced
fragments multiply-aligned to them. While these target sequences will
often be gene isoforms, they need not be. Haplotypes can be used as the
reference for allele-specific expression analysis, binding regions for
ChIP-Seq, or target genomes in metagenomics experiments. eXpress is
useful in any analysis where reads multi-map to sequences that differ in
abundance.
There are three methods to install berkeley-express on Ubuntu 22.04. We can use apt-get, apt and aptitude. In the following sections we will describe each method. You can choose one of them.
Install berkeley-express Using apt-get
Update apt database with apt-get using the following command.
sudo apt-get update
After updating apt database, We can install berkeley-express using apt-get by running the following command:
sudo apt-get -y install berkeley-express
Install berkeley-express Using apt
Update apt database with apt using the following command.
sudo apt update
After updating apt database, We can install berkeley-express using apt by running the following command:
sudo apt -y install berkeley-express
Install berkeley-express Using aptitude
If you want to follow this method, you might need to install aptitude first since aptitude is usually not installed by default on Ubuntu. Update apt database with aptitude using the following command.
sudo aptitude update
After updating apt database, We can install berkeley-express using aptitude by running the following command:
sudo aptitude -y install berkeley-express
How To Uninstall berkeley-express on Ubuntu 22.04
To uninstall only the berkeley-express package we can use the following command:
sudo apt-get remove berkeley-express
Uninstall berkeley-express And Its Dependencies
To uninstall berkeley-express and its dependencies that are no longer needed by Ubuntu 22.04, we can use the command below:
sudo apt-get -y autoremove berkeley-express
Remove berkeley-express Configurations and Data
To remove berkeley-express configuration and data from Ubuntu 22.04 we can use the following command:
sudo apt-get -y purge berkeley-express
Remove berkeley-express configuration, data, and all of its dependencies
We can use the following command to remove berkeley-express configurations, data and all of its dependencies, we can use the following command:
sudo apt-get -y autoremove --purge berkeley-express
References
Summary
In this tutorial we learn how to install berkeley-express package on Ubuntu 22.04 using different package management tools: apt, apt-get and aptitude.
